Black tea (Camellia sinensis) is one of the most popular and commonly consumed beverage worldwide possessing many chemopreventive properties. In the present set of investigations antimutagenic potential of Aqueous black tea extract (ATE) and Black tea polyphenols (BTP) was evaluated. Antimutagenic activity profile of ATE and BTP was notified towards Benzo(a)pyrene [BaP] and Cyclophosphamide [CP] in Salmonella typhimurium TA 98 and TA 100 tester strains. The induction of his+ revertant colonies by above mutagens was found to be inhibited by black tea. The frequency of chromosomal aberrations, micronucleus formation, and SCE’s induction by BaP/CP was reduced by ATE/ BTP administration in bone marrow cells of Swiss albino mice. The anticytotoxic potential of ATE and BTP was also evidenced by increase in the status of mitotic index. In addition black tea also exhibited protection in germ cell in dominant lethal mutation assay. Decrease in the number of living implants by BaP and CP was found to show increment by ATE and BTP treatment. The post implantation losses induced by BaP/CP were found to be reduced by ATE/BTP. The frequency of dominant lethal mutations induced by BaP/CP in different mating weeks was notified to inhibited by ATE/BTP. Hence the study depicts that black tea has potential in suppressing mutagenicity and cytotoxicity in microbial and mammalian somatic and germinal test systems.