In this dissertation it has been tried to evaluate the antitumour activity of Indole-3-carbinol in the living beings. For this purpose Swiss albino mice were selected. This study was conducted in Industrial toxicology & research center, LUCKNOW. Detailed investigations involving tumour developing techniques & histological techniques were used to demonstrate I3C action.

          The antimutagenic, anti-tumourogenic & anti-tumour promoting activity were studied using tumourogenic & tumour promoting chemicals. These chemicals included 7, 12dimethylbenz (a)anthracene(DMBA), Benzo(a) Pyrene(BaP),Cyclophosphamide(CP) & 12-0,tetra decanoylphorbol-13acetate(TPA). Along with this cytotoxity of I3C was observed on Ehrlich ascites cells. BaP & DMBA are widely occurring Polycyclic aromatic hydrocarbons. CP is prominently used anti-cancer drug showing its effect on cells due to its mutagencity. To show anti-mutagenicity & anti-genotoxicity of I3C CPwas used as a positive control.Here study was conducted on bone marrowcells of mice. CP has been shown to Cross Link with DNA both in vitro & invivo & thus induces point mutations. CP directly affect cell divison by inhibiting Mitotic index. I3C also mildly shows inhibition of Mitotic Index. But when given with CP shows decrease of cytotoxicity of CP.

          Swiss albino skin model was used to show antitumourogenic activity of I3C when it was applied topically on skin either one hour prior or one hour after carcinogen administration . I3C showed tumour initiation in both methods but it was more pronounced when it was applied after carcinogen application. Parameters used to show this were tumor induction time ,average tumour per mouse ,percentage of animals with tumour and cumulative number of tumours. For anti tumour promotion activity of I3C two stage skin model was used . A sub carcinogenic dose of DMBA was applied on skin then I3C was applied topically prior to application of TPA a well known tumour promoter. Here also the parameters were the same as in previous experiment which demonstrated antitumourogenic activity of I3C. I3C has been shown to increase life span of Ehrlich ascites tumour bearing mice.It also showed cytotoxicity against EA cells.These investigations and experiments showing anticarcinogenic& antimutagenic potential of I3C involving an array of skin tumour model, transplantable tumour model& mutagenicity test have provided important information that I3C can be used as adjuvant for cancer chemoprevention.