Production of oilseeds in recent years has fallen short of the requirement due to a number of reasons. One of the causes for low production is the inadequate attention given to the nutritional requirements of oilseed crops. In addition to nitrogen, another major nutrient, which has been attributed to have a multiple role in oilseed crop nutrition is sulphur. As such, sulphur deficiency in soils where these crops are raised, is considered as a major factor responsible for low oilseed production in India. Several authors are of the opinion that oilseeds not only respond to applied S but their requirement for sulphur is also the highest among crop plants. Further, low availability of S in soil limits the N use efficiency. There are a few reports showing that the two nutrients are closely related, synergistic and of vital importance for plants, because these are the major constituent of amino acids which constitute the building block of protein. These studies are able to demonstrate the essentiality of sulphur nutrition and interaction between S and N in optimizing seed and oil yield in rapeseed-mustard, but they are insufficient to provide a basis for evolving a nutrient management technology. Hence, an attempt was made in the present study to investigate the role of sulphur fertilization on nitrogen accumulation and yield of rapeseed-mustard through an array of biochemical parameters. In this study two sources of sulphur, viz. gypsum (conventional source) and soluble sulphur glass frits (SGF, non-conventional source) were taken. It has been found that sulphur application significantly improved the nitrogen accumulation in rapeseed-mustard when compared to control (no sulphur). This improvement in nitrogen accumulation is due to the improvement in nitrate reductase activity in the leaves of rapeseed-mustard. Sulphur application not only improved the N accumulation in plants, but also the partitioning of N toward economic part i.e. seed as evident from the higher nitrogen harvest index of the rapeseed-mustard grown with sulphur treatments. Among the sulphur treatments, application of 40 kg S ha^-1 in the form of gypsum in split doses and 30 kg S ha^-1 in the form of SGF in single dose were better in improving the N accumulation as well as the seed and oil yield, and quality. On the basis of the physiological and biochemical parameters which have their direct bearing on improvement in the yield of rapeseed-mustard, a nutrient management protocol was developed. This nutrient management protocol includes S, either applied at the rate of 40 kg S ha^-1 in the form of gypsum in split doses or 30 kg S ha^-1 in the form of SGF in single dose, for both the crops along with nitrogen, phosphorus and potassium applied at the rate of 100, 40 and 40 kg ha^-1, respectively. In this protocol, phosphorus and potassium were applied in a single basal dose at the time of sowing, while nitrogen at the rate of 100 kg N ha^-1 in two equal split doses.