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Thesis Summary



TITLE:

          Characterization and Standardization of Some Traditional Plant Drugs



Subject : Botany
Faculty : Botany
Name of the student : Sageer Ahmad Khan
Name of the supervisor : Dr. Javed Ahmad
Source of Funding : Ministry of Environment &   Forests & Indian Council of   Agricultural Research   (Government of India)
Date of Viva Voce : 27th March 2003


Summary

          In the following paragraphs the anatomical and phytochemical characters of root, leaf and flower drugs of six selected plants species have been described for the purpose of the standardization with their physical constants and pharmacognostical standards. There is resurgence of interest in ethnobotany and plants are reemerging as a significant source of new pharmaceuticals. The present research will certainly lead to systematic documentation of traditional knowledge and other botanical studies.

          Acacia leucophloea (Roxb.) Willd. (Mimosaceae) is a small or medium sized deciduous tree upto 6-8 meter in height. It commonly occurs in west land and open forest. The plant is highly medicinal having astringent, demulcent, aphrodisiac and anti-syphilitic properties.

         Macro and microscopic characters of the flowers of A. leucophloea show that there are numerous, globose, small, pale yellow, sweet scented heads. Solitary rhombohydron crystals are distributed in various regions of the peduncle, mainly in cortical parenchymatous cells and between the vascular bundles.

         Physico-chemical constants include: loss in weight up to 6.05% on drying at 105 °C. The solid content, crude fiber, total ash, water soluble ash, acid insoluble ash, sulphated ash and total alkaloids were reported up to 55.24%, 30.08%, 8.5%, 3.6%, 1%, 0.30% and 0.03% respectively.

         Sucrose (C12 H22 O11) was isolated from the flowers having m. p. 186-188 °C.

         Calliandra haematocephala Hassk. (Mimosaceae) is a bushy shrub upto1.5-4 m in height. It is distributed throughout tropical and sub tropical areas. The decoction product of the flowers is said to be blood purifier and also regarded as tonic.

         Macro and microscopic characters of the flowers of C. haematocephala are studied in detail. Pinkish red and scarlet powder puff like heads are very common. Pericycle contains a continuous and composite ring of 3-5 layers of hexagonal, thick walled sclerenchymatous cells in the peduncle zone.

         Physico- chemical constants show loss in weight on drying at 105 °C up to 10.69%. The solid content, crude fiber, total ash, water soluble ash, acid insoluble ash, sulphated ash, and total alkaloids were found up to 53.70%, 27.72%, 5.56%, 1.57%, 0.82, 0.02% and 0.06% respectively.

         Octacosane (C28H58) isolated from the flowers having m. p 60-620 C.

         Ehretia aspera Willd. (Ethretiaceae) is a shrub upto 3-5 m in height. It also occurs commonly in India. A decoction of the fresh roots is given in venereal diseases.

         Macro and microscopic characters of the leaves of E. aspera have been studied. The leaves are 3-10 x 2-6.5 cm in length, elliptical-oblong in shape, petiole 5-20 mm long. Stomata of anomocytic type, stomatal index (15), vein termination numbers (9-15), vein islet numbers (10-18) and palisade ratio (6-10) have been recorded.

         Physico- chemical constants show loss in weight up to 85.3% on drying at 105 °C. Crude fiber, total ash, water soluble ash, acid insoluble ash, sulphated ash and total alkaloids were measured and reported up to 49.5%, 10.67%, 2.57%, 1.06% 0.51% and 0.06% respectively.

         b-amyrin isolated from the leaves of this plant having m.p 196-1980C.

         Leucaena leucocephala (Lamk.)Wit. (Mimosaceae) is a large erect shrub over small tree upto 1.8-9.0 m in height. It is distributed in tropical regions. The bark of this plant is eaten for internal pain in Assam. The leaves are a good source of protein and carotene.

         Macro and microscopic characters of the leaves are studied. The leaves are bipinnate, 10-25 cm long, cauline and ramal. The taste is bitter and its odour is characteristic, stomatal index (12-20) and vein termination numbers (3-6) are recorded. The vascular bundles are arranged in horseshoe shape manner as shown in the transection of the rachis.

         Physico- chemical constants show loss in weight up to 91.4% on drying at 105 °C. The solid content, crude fiber, total ash, water soluble ash, acid insoluble ash, sulphated ash and total alkaloids were reported 49.9%, 37.39%, 11.27%, 4.87%, 1.02%, 0.53% and 0.05%.

         The impure compound from the leaves could not be characterised.

         Trianthema portulacastrum Linn.(Aizoaceae) is a diffuse, prostrate, branched herb, upto 30-65 cm long. It is found mostly in tropical regions. It is attributed with analgesic, antipyretic, anti-inflammatory, CNS depressant and stomachic properties and used in asthma, bronchitis, jaundice and oedema.s

         Macro and microscopic characters of the root of T. portulacastrum (white variety) are studied. The roots are small, fusiform, ranging between 8-18 cm in length. The Colour is yellowish white, odour is pungent and bitter in taste. Cluster crystals occur in some of the cortical cells of the root. Anomalous structure is also observed.

          Physico-chemical constants show loss in weight up to 5.5% on drying at 105 °C. The solid content, crude fiber, total ash, water soluble ash, acid insoluble ash, sulphated ash and total alkaloids were reported 35.6%, 41.5%, 10.5%, 7.7%, 1%, 0.51% and 0.05%.

          The compounds isolated from the root are b-sitosterol, stearic acid, palmitic acid and potassium nitrate.

          Mallotus philippinensis Muell Arg. (Euphorbiaceae) is an evergreen shrub and found throughout the tropical regions of India. Kamala powder alone is applied over syphilitic ulcer. It is also used as an anthelmintic, vermifuge and purgative medicine.

          Macro and microscopic characters of the commercial sample of the drug under the name kamala show that it is a granular brick-red powder. Microscopic examinations of powder reveal that it is a fine, purplish red or brick red powder consisting of no cellular structure.

          Physico-chemical constants show that the maximum percentage of the extract is found in chloroform (13.7%) and minimum in methanol (3.12%). Loss in weight on drying at 105°C was found up to 4.31%. The solid content, total ash, water soluble ash, acid insoluble ash and Sulphated ash were found to be 90.85%, 53.51%, 0.17%, 45.72%, and 20.55%.

          Rottlerin has been found in dark brown rhombic crystals, m. p.203-205 °C.